Jingya Ruan, Zheng Li, Ying Zhang, Yue Chen, Mengyang Liu, Lifeng Han, Yi Zhang, and Tao Wang

Abstract

Four new phenolic components, eurylophenolosides A (1) and B (2), eurylolignanosides A (3) and B (4), along with twelve known compounds were isolated from the roots of Eurycoma longifolia Jack. The structure of these components was elucidated by using various spectral techniques and chemical reactions. Among the known isolates, syringaldehyde (12), 3-chloro-4-hydroxybenzoic acid (13), 3-chloro-4-hydroxyl benzoic acid-4-O-ß-d-glucopyranoside (14), and isotachioside (15) were isolated from the Eurycoma genus for the first time. Further, the NMR data of 14 was reported here firstly. Meanwhile, the nitric oxide (NO) inhibitory activities of all compounds were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 40 µM. As results, piscidinol A (6), 24-epi-piscidinol A (7), bourjotinolone A (10), and scopoletin (16) were found to play important role in suppressing NO levels without cytotoxicity. Furthermore, the Western blot method was used to investigate the mechanism of compounds 6, 7, 10, and 16 by analysing the level of inflammation related proteins, such as inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) in LPS-stimulated RAW264.7 cells. Consequently, compounds 6, 7, 10, and 16 were found to significantly inhibit LPS-induced protein expression of IL-6, NF-?B and iNOS in NF-?B signaling pathway. Moreover, it was found that the protein expression inhibitory effects of 6, 7, and 16 exhibited in a dose-dependent manner. The mechanism may be related to the inhibition of the iNOS expressions through suppressing the IL-6-induced NF-?B pathway.

References:

Bhat, Rajeev; Karim, A.A. (2010), Tongkat Ali (Eurycoma longifolia Jack): A review on its ethnobotany and pharmacological importance, Fitoterapia, Volume 81, Issue 7, Pages 669-679

Chua, L.S.; Lau, C.H.; Chew, C.Y.; Dawood, D.A.S. (2019), Solvent Fractionation and Acetone Precipitation for Crude Saponins from Eurycoma longifolia Extract Molecules, Volume 24, Pages 1416

Henkel, Ralf R.; Wang, Ruxiang; Bassett, Susan H.; Chen, Tao; Liu, Na; Zhu,Ying ; Tambi, Mohd Ismail (2013), Tongkat Ali as a Potential Herbal Supplement for Physically Active Male and Female Seniors—A Pilot Study, Phytotherapy Research,Volume 28, Issue 4, Pages 544-550

Lahrita, Lucy; Hirosawa, Ryota; Kato, Eisuke; Kawabata, Jun (2017), Isolation and lipolytic activity of eurycomanone and its epoxy derivative from Eurycoma longifolia, Bioorganic & Medicinal Chemistry, Volume 25, Issue 17, Pages 4829-4834

Nhan NH, Loc NH. (2017), Production of eurycomanone from cell suspension culture of Eurycoma longifolia , Pharmaceutical Biology Vol 55(1) Pages 2234-2239

Rehman, Shaheed Ur; Choe, Kevin; Yoo, Hye Hyun (2016) Review on a Traditional Herbal Medicine, Eurycoma longifolia Jack (Tongkat Ali): Its Traditional Uses, Chemistry, Evidence-Based Pharmacology and Toxicology, Molecules, Vol 21(3), Pages 331, https://doi.org/10.3390/molecules21030331

Tong KL, Chan KL, AbuBakar S, Low BS, Ma HQ, Wong PF. (2015), The in vitro and in vivo anti-cancer activities of a standardized quassinoids composition from Eurycoma longifolia on LNCaP human prostate cancer cells, PLoS One. Vol 10(3)

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